Oxoid to Offer the DuPont Qualicon BAX® System Real-Time PCR Assay for Vibrios

Oxoid, a world leading microbiology brand, today announced that the DuPont Qualicon BAX® System Real-Time PCR Assay for Vibrios is now available alongside the Oxoid range in Europe, Canada, Australia and New Zealand.

Oxoid to Offer the DuPont Qualicon BAX® System Real-Time PCR Assay for Vibrios

The BAX® System Real-Time PCR Assay for Vibrio detects low levels of three distinct species – V. cholerae, V. parahaemolyticus and V. vulnificus – from the same sample, in less than 24 hours.

Tested on shrimp, tuna, oysters, scallops and crab, the BAX® System Vibrio Assay delivers 100 percent inclusivity for the three target species (42 V. cholerae, 20 V. parahaemolyticus, 29 V. vulnificus) and 100 percent exclusivity for non-target species (35 non-Vibrio; 20 non-V. cholerae, V. parahaemolyticus, V. vulnificus). Reliable, differentiated results are now available in less than one day, with the assay’s performance being equivalent to or better than the FDA Bacteriological Analytical Method (BAM) reference culture method, which typically takes three to five days.

This next-day test for Vibrio will be of great benefit to seafood companies, allowing them to improve operational efficiencies and make product release decisions more quickly and with confidence.

The BAX® System Real-Time Vibrio Assay procedure is simple to perform: stomach or blend 25g sample with 225ml alkaline peptone water, pre-warmed to incubation temperature; incubate samples at 35 ± 2°C for 18-20 hours; heat 5µl enrichment in prepared lysis reagent at 37°C for 20 minutes, then at 95°C for 10 minutes, cool for 5 minutes; hydrate PCR tablets with 30µl of lysate then load the rack into BAX® System instrument and start the test.

Results can be viewed and easily interpreted after just 90 minutes.

For further information about the BAX® System and assays available from Oxoid in Europe, Canada, Australia and New Zealand, please speak to your local Oxoid representative or contact Oxoid: tel +44 1256 841144, fax +44 1256 329728, email [email protected] or visit www.oxoid.com.