PCR-based assays

Improving the reliability of microbiological testing

3 January 2022 | By Andy Muirhead, Benjamin Diep, Benjamin Junge, François Bourdichon

Following Food Integrity’s 2021 panel around improving the reliability of microbiological testing, the experts come together again to recap the highlights.

It’s good to talk – communication between labs and customers

15 December 2020 | By Patrick McNamara

Patrick McNamara, Technical Specialist Manager, Intertek, provides a clear and concise overview of what information needs to be shared between labs and their customers.

User guide: SureTect Assay Workflow Quick Reference Guide

16 September 2020 | By Thermo Fisher Scientific

This Quick Reference Guide is intended as an overview of the Thermo Scientific™ SureTect™ Assay workflow only.

Study report: Thermo Scientific SureTect E. coli O157:H7 PCR Assay Method Extension For Use With the Applied Biosystems QuantStudio 5 RealTime PCR Instrument AOAC-RI PTM Validation: Method Comparison Study

16 September 2020 | By Thermo Fisher Scientific

The data presented in this report show that the SureTect E. coli O157:H7 PCR Assay is suitable for the detection of E. coli O157:H7 from a variety of food samples when using the QuantStudio 5 Real-Time PCR Instrument and associated RapidFinder Analysis Software.

Study report: Thermo Scientific SureTect Salmonella Species PCR Assay Method Extension For Use With the Applied Biosystems QuantStudio 5 Real-Time PCR Instrument AOAC-RI PTM Validation: Method ComparisonStudy

15 September 2020 | By thermo fisher, Thermo Fisher Scientific

The data presented in this report show that the SureTect Salmonella species PCR Assay is suitable for the detection of Salmonella species from a variety of food and environmental surface samples when using the QuantStudio 5 Real-Time PCR Instrument and associated RapidFinder Analysis Software.

Study report: Thermo Scientific SureTect Listeria species PCR Assay and Thermo Scientific SureTect Listeria monocytogenes PCR Assay method extension for use with the Applied Biosystems QuantStudio 5 RealTime PCR Instrument AOAC-RI PTM Validation: Method Comparison Study

15 September 2020 | By Thermo Fisher Scientific

This report details the method comparison study between the candidate methods and the International Organisation for Standardisation (ISO) 112901:2017 for a representative range of matrices.

Application note: Use of an Antibiotic-free Enrichment Medium for detection of salmonella in cheese samples using the Thermo Scientific SureTect Salmonella Species PCR Assay

14 September 2020 | By Thermo Fisher Scientific

This application note explores the use of the Thermo Scientific SureTect Salmonella Species PCR Assay for detection of salmonella in cheese samples.

Consumer food testing devices: threat or opportunity?

23 February 2018 | By Dr Bert Popping - FOCOS, François Bourdichon - International Dairy Federation (FIL-IDF)

Technological advances are bringing regular consumer testing of food for quality and safety closer to reality. Food safety consultant François Bourdichon, and Bert Pöpping, of FOCOS, discuss the implications of consumer testing for the industry.

Hygiena

Whitepaper: Bringing the Power of PCR DNA Identification to Food Safety

1 February 2018 | By Hygiena

When the polymerase chain reaction (PCR) was discovered nearly 35 years ago, it ushered in a revolution in biology.

Detector can spot traces of horsemeat in one hour

15 September 2017 | By New Food

Methods of testing raw meat DNA in lab conditions can often fail to differentiate between species. A Spanish research team has set out to tackle that problem.

New way to detect Palmer amaranth in contaminated seedlots

7 June 2017 | By New Food, University of Illinois College of Agricultural Consumer and Environmental Sciences (ACES)

Palmer amaranth, the aggressive and hard-to-kill weed, has established itself in droves. As a possible solution, some states declared Palmer a noxious weed, which prohibits its sale and transport.

Loop-mediated isothermal nucleic acid amplification (LAMP) for food microbiology testing

2 April 2017 | By Nigel Cook - Fera, Rosario Romero - Fera

Contamination of food products with pathogenic microorganisms is a major concern for food safety. The issue is exacerbated by modern production systems where high volumes, high speed and global distribution are common features. In order to protect consumers, regulations and procedures are in place to control microbiological hazards in the…

DuPont BAX System real-time PCR assay for Salmonella receives AOAC-RI validation extension

24 September 2015 | By Victoria White

The AOAC-RI has approved a method extension for the DuPont BAX System real-time PCR assay for Salmonella to include enrichment protocols using Actero Salmonella Enrichment Media from FoodChek Systems...

Viability PCR – the next level in PCR-based pathogen testing

29 October 2014 | By Dr. Marcia Armstrong

This recorded webinar introduces viability PCR as a fast and powerful tool to analyze food samples for the presence of potentially harmful microbes...