Application Note: A fast and robust GC/MS/MS analysis of 203 pesticides in 10 minutes in spinach Supported by: Published: 22 July 2022 Share this post 1 Share via Facebook Like Twitter Pinterest LinkedIn Digg Del Tumblr VKontakte Print Email Reddit Buffer Love This Weibo Pocket Xing Odnoklassniki iconOdnoklassniki WhatsApp Meneame Blogger Amazon Yahoo Mail Gmail AOL Newsvine HackerNews Evernote MySpace Mail.ru Viadeo Line Flipboard Comments Yummly SMS Viber Telegram Subscribe Skype Facebook Messenger Kakao LiveJournal Yammer Edgar Fintel Mix Instapaper Copy Link This application note describes two approaches for achieving a robust, multiresidue pesticide analysis in 10 minutes by GC/MS/MS for over 200 pesticides in spinach. This application note describes two approaches for achieving robust, multiresidue pesticide analysis in 10 minutes by GC/MS/MS, while maintaining sufficient chromatographic resolution for the analysis of over 200 pesticides in spinach; a challenging high chlorophyll, fresh matrix. First, the conventional 15 × 15 m (0.25 mm × 0.25 µm) midcolumn backflush configuration was used with an accelerated oven ramp, yielding an analysis time of 10 minutes. Second, a minibore 10 × 10 m (0.18 mm × 0.18 µm) midcolumn backflush configuration was used, enabling a fast 10-minute analysis time. The latter method was precisely scaled using the Agilent GC method translation technique. It was shown that midcolumn backflushing enabled method robustness and extended maintenance-free operation of the system by minimizing column trimming and source cleaning. Results demonstrate that the Agilent 7000E and 7010C triple quadrupole GC/MS systems delivered excellent linearity over a concentration range of 0.1 to 1,000 parts per billion (ppb). Method robustness was shown with 700 consecutive injections of a spinach extract, spiked with pesticides at 20 ppb, that spanned over 175 hours of continuous running of the GC/TQ.
